Use the split segment clause to divide a segment in 2 or more parts.
When creating a new segment clause select the "Split" clause.
Next, assign however many groups this segment should be split into.
Select which group this segment should pull.
When you are ready to create the segment for the next group, simply copy the first segment and change the group criteria to say 'Take the 2nd' (or 3rd, or 4th, etc.).
Split Segmentation is not enabled by default for any account. If you would like to use this feature please send a request to your Client Success Manager or support.